ABSTRACT
Objective To construct the eukaryotic expression vector for human hypoxia inducible factor-1? gene (pSNAV-HIF-1?), and to investigate the apoptosis and intracellular calcium concentration of the transfected A?25-35 induced hippocampal neurons of primary culture. Methods Human hypoxia inducible factor-1? gene from pBSKhHIF1?T7 was inserted into pSNAV2.0 by the method of gene recombination, then the constructed vector was transfected into hippocampal neurons of primary culture and followed by A?25-35 for treatment. Empty pSNAV2.0 vector was treated the same way as pSNAV-HIF-1? as a control. The expression level of HIF-1? protein was assayed by Western blotting. Apoptosis was detected by flow cytometry. Intracellular calcium concentration was determined by laser scanning confocal microscopy with Fluo-3/AM as the fluorescent dye. Results It was shown that pSNAV-HIF-1? was successfully constructed by restriction enzyme digestion, PCR and DNA sequencing. The expression level of HIF-1? protein was significantly increased in transfected hippocampal neurons of primary culture (P
ABSTRACT
5 cases of orthotopic live transplantation (OLTX) were performed in unresectable hepatic cellular carcinoma(3),Wilson's disease(1)and hepatitis B(1).The donor livers were harvested by the technique of rapid multiple organ harvesting,flushed and preserved with UW solution.The veno-venous bypass was introduced during the anhepatic phase in the operation.The double or triple-drug immunosuppressive regimen was used and three episodes of acute rejection were controlled in two patients.One patient died of CMV infection and another,of upper gastrointestinal hemorrhage on the 92nd and 58th postoperative day respectively.The other three are still alive for 35,150 and 210 days.
ABSTRACT
Objective To investigate the effects of two weaning modes of mandatory rate ventilation (MRV) and SIMV+PSV on respiratory mechanics in patients with type Ⅱ respiratory failure. Methods A total of 30 patients with type Ⅱ respiratory failure were sem randomly divided into two groups. Patients with type Ⅱ respiratory failure received ventilation in CMV mode or CMV/ACMV modes and then SIMV+PSV. When patients could be weaned, MRV mode was adopted in MRV group, but SIMV+PSV modes were adopted in the control group. After continuous operation in each mode for 60 min and when the tidal volume (VT) and minute ventilation (MV) in MRV mode were the same as those in SIMV+PSV mode, PIP, PP, Pm, PEEPi, blood gas changes, and weaning success in the two groups were observed during ventilation. Results PIP, PP, and Pm in patients in MRV mode were significantly lower than those in SIMV+PSV modes ( P 0.05), but the synchronism in patients in MRV group was better. Conclusion MRV is a more adaptable weaning mode for patients with type Ⅱ respiratory failure.